Journal: Communications Medicine

Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

doi: 10.1038/s43856-025-00870-2

Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProt™ microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Ophthalmology

Article Title: Calpastatin participates in the regulation of cell migration in BAP1-deficient uveal melanoma cells

doi: 10.18240/ijo.2019.11.03

Figure Lengend Snippet: A: Scannogram of the human proteome microarray (HuProt™ microarray) detecting interacting proteins with BAP1 and a partially enlarged lattice indicating the positive signal of CAST; B: Interactions between BAP1 and CAST proteins by surface plasmon resonance analysis: ① the original graph (upper) and the weighted graph (lower) showing the binding kinetics for the interaction between CAST and BAP1 over time; ② legend showing dilutions of BAP1 protein; ③ table listing the dissociation constant (KD value, 7.42×10−9) between these two proteins; C, D: IPs in OCM-1A cells to detect the protein interaction between BAP1 and CAST, as well as BAP1 and vinculin.

Article Snippet: To screen for proteins interacting with BAP1, we used the HuProt™ microarray (CDI Laboratories, Inc., Mayaguez, Puerto Rico), which is composed of approximately 20 000 human full-length proteins with N-terminal glutathione S-transferase (GST) tags.

Techniques: Microarray, SPR Assay, Binding Assay

Journal: bioRxiv

Article Title: PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner

doi: 10.1101/2024.04.11.589075

Figure Lengend Snippet: (a) Distribution of PAK6 candidate interactors according to their Z-score retrieved from a Human Proteome Microarray probed with recombinant full-length human PAK6. (b) A GO:BP analysis using gProfiler g:GOSt ( https://biit.cs.ut.ee/gprofiler/gost ) was performed for PAK6 candidate interactors with Z score >2.5 (left) and for PAK6 interactors annotated in PPI web-based tools PINOT, HIPPIE and MIST (PHM) (right). GO:BP terms with 2000 (array) and 1000 (PHM) term size were grouped into semantic categories. (c) Venn diagrams showing overlaps between the primary cilium proteome (GO:0005929, 640 genes) and the experimental (array) PAK6 interactome (left) or the literature-based (PHM) PAK6 interactome (right). (d) Protein network of overlapping PAK6 interactors with the primary cilium proteome (c) (including PAK6) obtained with STRING ( https://string-db.org/cgi/input?sessionId=b1S4T5BW27rz&input_page_show_search=on ); number of nodes: 11, number of edges: 11, average node degree: 2, average local clustering coefficient: 0.591, expected number of edges: 3, PPI enrichment P -value: 0.000502. Blue nodes are ciliary proteins present in the experimental PAK6 interactome (array) and grey nodes are those found in the literature-based PAK6 interactome.

Article Snippet: HuProt TM Human Proteome Microarray v4.0 was purchased from Cambridge Protein Arrays (Babraham Research Campus, Cambridge, UK) and employed to screen PAK6 interactor candidates following manufacturer’s instructions.

Techniques: Microarray, Recombinant

Journal: International Journal of Molecular Sciences

Article Title: Identification of Tumor Antigens in Ovarian Cancers Using Local and Circulating Tumor-Specific Antibodies

doi: 10.3390/ijms222011220

Figure Lengend Snippet: Antigen reactivities identified with ovarian cancer cohort 1 using Sengenics CTA protein arrays. Ovarian cancer ASC probes ( n = 11, A–K) and selected matched sera ( n = 5) were screened using Sengenics CTA protein arrays.

Article Snippet: Screening the ASC probes on two high-density protein microarrays, the CDI HuProt TM array and the Sengenics CTA protein array, led to the identification of 41 candidate cancer-specific antigens.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Identification of Tumor Antigens in Ovarian Cancers Using Local and Circulating Tumor-Specific Antibodies

doi: 10.3390/ijms222011220

Figure Lengend Snippet: Study design diagram. Ovarian cancer cohort 1 and selected healthy and benign controls were screened using the CDI HuProt TM array and the Sengenics CTA protein array, which led to the identification of 50 candidate antigens. A custom array was developed and used to screen ovarian cancer cohort 2, as well as healthy and benign controls.

Article Snippet: Screening the ASC probes on two high-density protein microarrays, the CDI HuProt TM array and the Sengenics CTA protein array, led to the identification of 41 candidate cancer-specific antigens.

Techniques: Protein Array